Generator
Part:BBa_K318023:Design
Designed by: Nathaniel Pantalone, Justin Vrana Group: iGEM10_Wisconsin-Madison (2010-07-13)
pLac + RBS + Int (P22)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 498
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.
Source
biobricks